Antibodies

Key requirements for a successful generation of Abs include antigen design, immunization strategies and different screening technologies. We have a great care in the design of antigens; we can synthesize a peptide or, we can produce the full lenght proteins. From research amount supplies to industrial production of Abs, the immunization and sampling schedule are customized to meet sponsor specifications.

 

MONOCLONAL (mAb)

Immunization (protein, peptide and cellular Ag)
Screening of serum
Fusion
Primary Screening of hybridomas
Reconfirmation screening
Subcloning
Hybridoma stabilization
Hybridoma Banks
Purification
Characterization (subclass mAb, epitope mapping,
WB, ELISA, HPLC, IEF, SDS-PAGE)
Large-scale production and purification

 

The quantities, biochemical or molecular characterizations, the number of clones to be generated depend on  customer's requests. Antigen may be generated in-house by the Protein Factory, starting from cDNA

 

POLYCLONAL (pAb)

Production of policlonal antibodies in rabbits
Dedicated immunization protocols (protein, peptide and cellular Ag)

Screening of serum
Antibodies purification
Antibodies characterization