Experimental Pharmacology

&

Analytical Biochemistry

This unit supplies biochemical and cell-based screenings aimed to assess the biological activity and toxicity profile of small molecules and/or biologics.
The custom cell-based assays and the high-throughput screening (HTS) platforms allow to rapidly characterize the test compound in terms of mechanism of action, cytotoxicity, and other properties.

 

Experimental Activities

Cell culture (maintenance and storage, tests for pathogens and toxins identifications)

Isolation of new primary cell lines

Cytotoxicity and proliferation assays

XC50 testing and confirmations

Signal transduction assays

Reporter gene assays

Cytokine release assays

FACS

Chemotaxis, migration and angiogenesis

Live Cell Imaging (Operetta High-Content Imaging System)

Evaluation of ligand-receptor affinity by SPR (Biacore T200)

Immunoassays (ELISA, Western blot, Immunofluorescence)

On the basis of the experimental requirements it is possible to choose between more than 100 cellular models, including:

stabilized cell lines

primary cells from different tumour types

unique pre-clinical models of malignant mesothelioma

engineered cell lines expressing  GFP or Luciferase reporter genes